Fluorescent nucleobase analogues for base–base FRET in nucleic acids: synthesis, photophysics and applications

• Mattias Bood,
• Sangamesh Sarangamath,
• Moa S. Wranne,
• Morten Grøtli and
• L. Marcus Wilhelmsson

Beilstein J. Org. Chem. 2018, 14, 114–129, doi:10.3762/bjoc.14.7

• DNA display large Stokes shifts, cover a broad wavelength region and the maxima are found at 505 and 450 nm (Figure 3), respectively [30][31]. Their spectral envelopes, which are an important factor for the overlap integral in FRET, are robust to changes in the local environment. The fluorescence

DNA display of glycoconjugates to emulate oligomeric interactions of glycans

• Alexandre Novoa and
• Nicolas Winssinger

Beilstein J. Org. Chem. 2015, 11, 707–719, doi:10.3762/bjoc.11.81

• programmability of the assemblies lends itself to a combinatorial display of multiple ligands. Recent efforts in the synthesis and applications of such conjugates are discussed. Keywords: glycan; glycoconjugate; DNA display; multivalency; nucleic acid conjugates; oligomeric interaction; Introduction Cell

• correlation between affinity and the inter-galactose distance thus establishing that DNA display of glycans can be used to tune the optimal spatial arrangement of the ligands in synergic interactions. This synthetic strategy was further refined by Seeberger and co-workers [22] with the use of commercially

• library can be reassembled making the technology compatible with reiterative cycles of selection/amplification. Seitz and co-workers demonstrated that a DNA display could be used to interrogate topologically more challenging geometries, namely, bridging binding sites on opposing faces of a lectin. Using a